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PMC:1472690 JSONTXT

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div. section text # proj. size # Ann.
0 TIAB β-(1->3)-D-glucan modulates DNA binding of nuclear factors κB, AT and IL-6 leading to an anti-inflam 33 2244 1673 show
1 Background β-1→3-D-glucans occur as a principal component of microbial cell walls or can be secreted from both, 26 3570 2091 show
2 Results Binding activities of NFκB, NFIL-6 and NFAT from human PBMC to the TNFα, IL-6 and IFNγ promoters fol 26 1559 1326 show
3 Results Cytokine profile of human PBMC following a 48 h in vitro stimulation with LPS, TSST-1, GP, GP + LPS 26 4196 3551 show
4 Results Role of NFκB, NFIL-6 and NFAT binding in LPS-, TSST-1-, GP-, GP + LPS- and GP + TSST-1-induced IL-1R 26 2304 1568 show
5 Results GP modulated the TSST-1-induced IL-1β/IL-1RA ratio towards an anti-inflammatory phenotype In a simpl 26 1142 904 show
6 Results Inhibition of transcription factors led to reduced IL-1RA levels Pharmacological inhibitors were use 26 1265 891 show
7 Discussion Animal models of sepsis and myocardial injury suggest that a β-1→3-D-glucan like GP mediates its pro 26 8213 5831 show
8 Conclusion In summary, our data demonstrated that in vitro glucan phosphate induced a transcription factor bind 26 761 381 show
9 Methods Materials Water soluble phosphorylated β-1→3-D-glucan (GP) was prepared as done before [20]. The phy 26 410 157 show
10 Methods Isolation of human PBMC Buffy coats were obtained from healthy blood donors in compliance with the H 26 741 315 show
11 Methods Stimulation conditions For induction of transcription factors and cytokines, PBMC were cultured in a 26 612 302 show
12 Methods Oligonucleotides (Oligos) and 5'-32-P-Labeling The complementary double-stranded (ds) oligonucleotid 26 972 539 show
13 Methods Preparation of nuclear extracts and electrophoretic mobility shift assays (EMSA) Nuclear extracts of 26 874 396 show
14 Methods Immuno (Dot) blot To determine whether the DNA binding proteins were related to NFκB and NFAT, dot b 26 1270 539 show
15 Methods Cytokine measurements Supernatants of cell cultures were harvested 3, 6, 9, 12, 18, 24, 30, 36 and 4 26 894 517 show
16 Methods Inhibition of transcription factors To inhibit translocation and binding of transcription factors to 26 1253 741 show
17 Methods Statistical analysis The Kolmogorow-Smirnov test was used to evaluate, whether cytokine amounts and 26 897 404 show
18 Caption-Figure 1 A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or 26 1245 934 show
19 Caption-Figure 2 NFκB p65/p50 and NFATc2/c1 were involved in GP-induced DNA binding. In order to determine whether th 26 565 547 show
20 Caption-Figure 3 A, IL-1β was down-regulated (12 h – 24 h) following GP + TSST-1 when compared to TSST-1. Time course 26 1204 997 show
21 Caption-Figure 4 GP modulated LPS- and TSST-1-induced binding to a new NFκB3 site in the IL-1RA promoter. Binding was 26 355 294 show
22 Caption-Figure 5 GP modulated TSST-1-induced DNA binding to a new NFATP2/3 site in the IL-1RA promoter. Binding was u 26 773 595 show
23 Caption-Figure 6 Decreased IL-1β/IL-1RA ratio following GP + TSST-1 when compared to TSST-1. Time course (48 h) of th 26 727 697 show
24 Caption-Figure 7 Inhibition of GP-induced NFκB, NFAT and NFIL-6 DNA binding to the IL-1RA promoter. A, A representati 26 910 730 show
25 Caption-Table 1 Sense strand of oligonucleotides used in EMSAs. 26 47 17 show
26 Caption-Figure 8 Cartoon of the sense strand of the IL-1RA promoter. Relative locations of previously (LRE-1) [30-33] 26 230 109 show