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PMC:1310901 JSONTXT

Divisions (16) JSON TSV

div. section text # proj. length # Ann.
0 TIAB Down-regulation of interferon regulatory factor 4 gene expression in leukemic cells due to hypermeth 26 1785 901 show
1 INTRODUCTION Chronic myeloid leukemia (CML) is a clonal myeloproliferative disorder with a typical three phased c 20 3316 1799 show
2 MATERIALS AND METHODS Cell lines K-562, Jurkat and U-937 were obtained from the ATCC (American Type Culture Collection, Ro 20 339 156 show
3 MATERIALS AND METHODS Cell culture and stimulation All cell lines were maintained at 5% CO2 in RPMI 1640 medium with 1% gl 20 478 190 show
4 MATERIALS AND METHODS Sequencing of the IRF-4 promoter For analysis of the IRF-4 promoter region for permanent aberrations 20 835 316 show
5 MATERIALS AND METHODS Expression analysis To analyze the IRF-4 transcriptional level, RNA was extracted from cells using t 20 1906 984 show
6 MATERIALS AND METHODS Methylation-specific restriction-PCR-assay DNA was extracted with a commercial kit (Qiagen) as recom 20 1483 582 show
7 MATERIALS AND METHODS Bisulfite treatment DNA was extracted as described above. Bisulfite treatment of DNA, leading to con 20 1852 722 show
8 MATERIALS AND METHODS In vitro methylation and reporter gene assays The IRF-4 promoter-reporter gene construct was generou 20 990 471 show
9 RESULTS Absence of IRF-4 expression in leukemia cells is not due to promoter alterations We have previously 20 1266 792 show
10 RESULTS Increase of IRF-4 expression in hematopoietic cells after demethylating treatment We next analyzed w 20 1644 875 show
11 RESULTS Methylation-sensitive enzymes do not cut specific sites in the IRF-4 promoter in hematopoietic cells 20 1562 689 show
12 RESULTS Specific CpG sites in the IRF-4 promoter are methylated in hematopoietic cells In order to exactly m 20 2216 1166 show
13 RESULTS In vitro methylation of an IRF-4 promoter-reporter construct decreases its activity To provide evide 20 1028 508 show
14 RESULTS mRNA expression of DNA methyltransferases and methyl-CpG-binding proteins may not be associated with 20 877 721 show
15 DISCUSSION Many genetic lesions are known to influence gene expression of tumor suppressor genes. Whereas mutat 20 6519 3705 show