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Divisions (21)
 

div. section text # proj. length # Ann.
0 TIAB Increased interleukin-17 production via a phosphoinositide 3-kinase/Akt and nuclear factor ?B-depend 34 2395 2073 show
1 Introduction Rheumatoid arthritis (RA) is characterized by infiltrations of macrophages and T cells into the join 27 3227 2608 show
2 Materials and methods Patients Informed consent was obtained from 24 patients (5 men and 19 women) with RA who fulfilled t 27 783 527 show
3 Materials and methods Reagents Recombinant IL-17, IL-18, IL-15, monocyte chemoattractant protein-1 (MCP-1), macrophage inf 27 832 701 show
4 Materials and methods Production of IL-17 by T cell receptor activation, cytokines or chemokines PBMC were prepared from h 27 1029 609 show
5 Materials and methods CD4+ T-cell isolation by MACS Anti-CD4 microbeads were used essentially as recommended by the manufa 27 1096 710 show
6 Materials and methods Enzyme-linked immunosorbent assay of IL-17 IL-17 in culture supernatants was measured by sandwich en 27 1480 940 show
7 Materials and methods Quantification of IL-17 mRNA by semiquantitative reverse transcription–polymerase chain reaction PBM 27 1592 1022 show
8 Materials and methods Western blot analysis of Akt, phosphorylated Akt and IκB-α PBMC were incubated with anti-CD3 (10 μg/ 27 1256 783 show
9 Materials and methods Gel mobility-shift assay of NF-κB binding site Nuclear proteins were extracted from about 5 × 106 PB 27 1146 780 show
10 Materials and methods Cell viability (Trypan blue dye exclusion assay) For cell viability assays, the trypan blue dye excl 27 394 231 show
11 Materials and methods Statistical analysis Data are expressed as means ± SEM. Statistical analysis was performed with Stud 27 184 93 show
12 Results IL-17 production in PBMC from patients with RA, patients with OA and normal individuals PBMC were se 27 625 537 show
13 Results Increased IL-17 production in PBMC of patients with RA by anti-CD3 and/or anti-CD28, and PHA Because 27 848 755 show
14 Results Regulation of IL-17 production in RA PBMC by inflammatory cytokines and chemokines Because RA PBMC i 27 931 923 show
15 Results Inhibition of IL-17 production by signal transduction inhibitors and anti-rheumatic drugs Having obs 27 1429 1113 show
16 Results IL-17 mRNA expression in RA PBMC To see whether enhanced IL-17 production could be regulated at a tr 27 390 302 show
17 Results Activation of PI3K/Akt signal transduction pathway on IL-17 production by anti-CD3 To determine down 27 829 772 show
18 Results Activation of the NF-κB and activator protein-1 (AP-1) pathway in the IL-17 promoter region To inves 27 1434 1349 show
19 Discussion IL-17 was first described as a T cell product with proinflammatory properties [5,22]. RA is characte 27 6083 5221 show
20 Conclusions We have detected a more pronounced production of IL-17 from RA PBMC in response to IL-15 and MCP-1 a 27 695 577 show