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Divisions (21)
 

div. section text # proj. length # Ann.
0 TIAB Increased interleukin-17 production via a phosphoinositide 3-kinase/Akt and nuclear factor ?B-depend 33 2395 2038 show
1 Introduction Rheumatoid arthritis (RA) is characterized by infiltrations of macrophages and T cells into the join 26 3227 2575 show
2 Materials and methods Patients Informed consent was obtained from 24 patients (5 men and 19 women) with RA who fulfilled t 26 783 527 show
3 Materials and methods Reagents Recombinant IL-17, IL-18, IL-15, monocyte chemoattractant protein-1 (MCP-1), macrophage inf 26 832 686 show
4 Materials and methods Production of IL-17 by T cell receptor activation, cytokines or chemokines PBMC were prepared from h 26 1029 608 show
5 Materials and methods CD4+ T-cell isolation by MACS Anti-CD4 microbeads were used essentially as recommended by the manufa 26 1096 705 show
6 Materials and methods Enzyme-linked immunosorbent assay of IL-17 IL-17 in culture supernatants was measured by sandwich en 26 1480 932 show
7 Materials and methods Quantification of IL-17 mRNA by semiquantitative reverse transcription–polymerase chain reaction PBM 26 1592 1010 show
8 Materials and methods Western blot analysis of Akt, phosphorylated Akt and IκB-α PBMC were incubated with anti-CD3 (10 μg/ 26 1256 777 show
9 Materials and methods Gel mobility-shift assay of NF-κB binding site Nuclear proteins were extracted from about 5 × 106 PB 26 1146 776 show
10 Materials and methods Cell viability (Trypan blue dye exclusion assay) For cell viability assays, the trypan blue dye excl 26 394 231 show
11 Materials and methods Statistical analysis Data are expressed as means ± SEM. Statistical analysis was performed with Stud 26 184 93 show
12 Results IL-17 production in PBMC from patients with RA, patients with OA and normal individuals PBMC were se 26 625 531 show
13 Results Increased IL-17 production in PBMC of patients with RA by anti-CD3 and/or anti-CD28, and PHA Because 26 848 744 show
14 Results Regulation of IL-17 production in RA PBMC by inflammatory cytokines and chemokines Because RA PBMC i 26 931 907 show
15 Results Inhibition of IL-17 production by signal transduction inhibitors and anti-rheumatic drugs Having obs 26 1429 1104 show
16 Results IL-17 mRNA expression in RA PBMC To see whether enhanced IL-17 production could be regulated at a tr 26 390 299 show
17 Results Activation of PI3K/Akt signal transduction pathway on IL-17 production by anti-CD3 To determine down 26 829 762 show
18 Results Activation of the NF-κB and activator protein-1 (AP-1) pathway in the IL-17 promoter region To inves 26 1434 1329 show
19 Discussion IL-17 was first described as a T cell product with proinflammatory properties [5,22]. RA is characte 26 6083 5152 show
20 Conclusions We have detected a more pronounced production of IL-17 from RA PBMC in response to IL-15 and MCP-1 a 26 695 569 show