As shown in Figure 3B , 3HP-β-LG (15 μg/ml) could fully inhibit HPV16 PsV entry when it was added to the cells 0.5 h before and 0.5, 1, 2, 4, and 8 h after addition of HPV16 PsV, respectively, while about 70, 10, and 5% of HPV16 PsV entry were blocked when 3HP-β-LG was added to the cells 12, 24, and 48 h after addition of HPV16 PsV, respectively ( Figure 3B ).